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The terms "relative fluorescence units" (RFU)〔 For more than a century, the term "RFU" has been associated with the Rugby Football Union, founded in 1871, and the term "RFU level" is common in that connection. 〕 and "RFU peak" refer to measurements in electrophoresis methods, such as for DNA analysis. A "relative fluorescence unit" is a unit of measurement used in analysis which employs fluorescence detection.〔 "Relative fluorescence unit (RFU)", DNA.gov: Glossary, April 2011, webpage: (DNA-gov-RFU ). 〕 Fluorescence is detected using a charged coupled device (CCD) array, when the labeled fragments, which are separated within a capillary by using electrophoresis, are energized by laser light and travel across the detection window. A computer program measures the results, determining the quantity or size of the fragments, at each data point, from the level of fluorescence intensity.〔 Samples which contain higher quantities of amplified DNA will have higher corresponding RFU values.〔 "Thresholds", NFSTC.org, 2010, webpage: (). 〕 An "RFU peak" is a relative maximum point along a graph of the analyzed data. The data can be normalized to DNA input or additional normalizing genes. The RFU heights can range from 0 to 2000 or more. ==DNA PCR analysis== The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary DNA target. Frequently, real-time PCR is combined with reverse transcription to quantify messenger RNA and Non-coding RNA in cells or tissues. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Relative fluorescence units」の詳細全文を読む スポンサード リンク
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